Maximizing DNA Yields from Peripheral Blood Mononuclear Cells for Archiving and NGS Analysis

Peripheral Blood Mononuclear Cells (PBMCs) are a crucial sample type for a broad range of research applications including profiling immune cells and serving as “normal” controls for studies of FFPE tumor tissue for oncology studies. Maximizing the DNA yield from PBMCs enables archiving and increases the number of downstream assays that can be performed. The Ionic Purification System from Purigen Biosystems leverages isotachophoresis to maximize the yield of DNA from PBMCs. During the webinar data will be presented showing significantly improved yields of DNA extracted from PBMCs ranging in cell count from 10 cells to 5 million cells.

Learning Objectives:
• Learn how DNA yield and quality impact downstream genomic analysis
• Understand how Isotachophoresis (ITP) works and enables superior nucleic acid extraction vs legacy technologies (columns, beads, precipitation, etc.)
• Evaluate data showing superior yields from ITP-based extraction
• Explore options for trying out ITP in your lab