Technique Talk: Improving IHC and IF Staining Results

Immunohistochemistry (IHC) and immunofluorescence (IF) are well established, trusted applications for investigating protein detection and localization within cell and tissue preparations. However, even experienced researchers sometimes struggle with recurring problems, such as background interference, lack of specificity, weak signal, or poor reproducibility. These challenges can compound, requiring valuable time and resources for troubleshooting, reducing lab productivity, and delaying the generation of meaningful results.

This Technique Talk sponsored by Vector Laboratories will explore approaches for optimizing your IHC and IF staining workflows.

Learning Objectives
• Selecting staining reagents
• Identifying and eliminating sources of non-specific staining
• Appropriate controls