Multi-View Light Sheet Microscopy to Track Cell Lineages in Tissue Morphogenesis

Explore the complex dynamics of cell fate decisions and morphogenesis through 3D time-lapse microscopy, using real user data to advance our understanding of developmental biology.
In this webinar, you will learn:
1. How to use multi-view light sheet microscopy to track cell lineages in vivo during embryonic development
2. Ways to use cell tracking data to form hypotheses on gene regulatory networks influencing pattern emergence during morphogenesis
3. About the unique versatility of the Viventis Deep microscope in studying the development of various species and embryo models

A major question in developmental biology is understanding how cellular fate decisions are regulated precisely in space and time.
Fortunately, we can now begin to observe these processes with modern imaging approaches, which allow the tracking of cell fate decision events and cellular rearrangements by live 3D time-lapse microscopy.

This development is shifting our understanding of pattern formation in development away from static models towards models based on the principles of dynamical systems and statistical mechanics.

Join Dr Andrea Boni for a live demonstration of how light-sheet microscopy is the technique of choice for volumetric live imaging of 3D samples at high speed with minimal phototoxicity.

Follow along with two user examples of how light-sheet microscopy is helping scientists understand the biological process of intestinal and brain organoid development.

After this demo, we will introduce the technology behind the Leica Viventis Deep, which combines dual-view illumination and detection in an open-top configuration.

Andrea will demonstrate how this unique configuration allows easy sample mounting and long-term imaging from small embryos and organoids to large multicellular systems.