MULTI-seq: Single-Cell Genomic Sample Multiplexing Using Lipid-Tagged Indices

While single-cell genomic technologies can assay many thousands of unique cells per experiment, their sample throughput—the number of analyzed unique samples—remains severely limited. This hampers experimental design potential, especially for mechanistic studies, which rely on sufficient sample replicates, perturbations, and spatiotemporal information to establish causality. Sample multiplexing through technologies like MULTI-seq address this limitation by labeling cells and/or nuclear membranes with sample-specific DNA barcodes prior to single-cell isolation, allowing multiple samples to be pooled and assayed simultaneously.

In this webinar brought to you by MilliporeSigma , Chris McGinnis and Jennifer Silverman will describe the advantages and applications of MULTI-seq using lipid-modified oligonucleotides, including single-cell epigenomics assays, spatial transcriptomics, and single-cell chemical screens.

Topics to be covered
• How MULTI-seq improves single-cell genomic throughput and data quality while reducing reagent costs
• How MULTI-seq compares to other sample multiplexing technologies
• The features and benefits of the MULTI-seq lipid-modified oligo kit