Slough is a well-known feature of chronic wounds. However, the tissue and microbial composition of slough are not well defined. Clinically, it’s difficult to define what is normal slough and identify wounds likely to heal versus deteriorate. Here I will discuss a recent project to comprehensively characterize the human and microbial components of slough. I will present data to suggest that slough itself is a potential biomarkers of wound healing. Collectively, these findings underscore how slough components can help identify wounds at risk of continued impaired healing. Future studies aim to explore these predicted biomarkers in a larger cohort.