Technique Talk: Purifying Plant-Based Endogenous Biomolecules

Nucleic acid isolation from plant tissues presents unique challenges. Commonly used solvent-based and silica-based extraction methods leave behind chaotropic salts and other inhibitors that negatively affect results in downstream applications, such as qPCR. Nucleic acid extraction from plants by negative chromatography provides better yield than traditional silica methods and reduces impurities, providing superior performance in downstream analyses.

In this Technique Talk, April Bauer will discuss negative chromatography-based purification for plant nucleic acids using GenElute™-E as well as sample preparation and PCR reagent selection for successful qPCR results.