Whole Organism Phenotyping to Identify Regulators of Choanoflagellate Development in an Academic Setting
Choanoflagellates are the closest living relatives of animals. These single-celled protists express diverse animal genes, such as kinases, and are therefore a compelling system for studying ancestral functions of animal gene families. The emergence of high throughput techniques in academic settings has opened new opportunities for scientists to profile and characterize such biological systems.
In this webinar, researchers from the University of California, Berkeley, and Stanford University show how choanoflagellates can be used in a core facility to identify novel regulators of cell biology and present a use case of how StratoMineR could be useful for data analytics. They show how their approach can enable the discovery of small molecule regulators of choanoflagellate biology, including those that inhibit cell proliferation and colony formation in Salpingoeca rosetta, a model choanoflagellate.
You will learn:
- How high-throughput screening can be implemented in an academic setting
- How to set up a high-throughput screen as a user in a core facility
- How StratoMineR can be used to identify hits from a high-throughput imaging screen
