Tips for Successful Single-Cell & Single Nucleus RNA-seq Experiments

Dr. Martelotto has been recently been appointed Scientific Director of the Single Cell Core (SCC) Laboratory of Harvard Medical School (HMS), Department of Systems Biology. Prior to joing HMS, was the Head of the Single Cell Innovation Laboratory (SCIL) at The University of Melbourne Centre for Cancer Research (UMCCR) within the Victoria Comprehensive Cancer Centre (AUS) and as such has spearheaded the development and single cell-based -omics. Amongst his ground-breaking achievements is the establishment of a single-cell analysis of fresh, frozen and archival (FFPE) human samples, which facilitated our understanding of intra-tumour genetic heterogeneity (Xue, Martelotto et al., Nature Medicine 2017; Martelotto et al., Nature Medicine 2017). His current team develops, implements and offers current methods of single cell genomics in order to build a bridge between researchers and single-cell technologies in their quest to understand how cells collectively perform systems-level functions in healthy and diseased states. Through collaborative efforts with leading Institutes in Single cell genomics and Spatial Transcriptomics such as the Peter MacCallum Cancer Centre, UMCCR, The Garvan Institute of Medical Research (AUS) and the Memorial Sloan Kettering Cancer Centrer (USA) as well as genomics companies like 10x Genomics and Illumina his team addresses how cellular heterogeneity shapes complex biological systems.