Multiplex protein expression analysis in tissue using immunohistochemistry has rapidly evolved over the last decade from 2- and 3-plex assays to a field of new technologies enabling highly multiplexed assays of 40 or more targets. Knowing which of these spatial proteomic technologies would be most effective in your lab or group is not simple. In this presentation we will walk through the value proposition for spatial proteomics, focusing on antibody- and immunohistochemistry-based spatial proteomics platforms, as well as review technical vignettes describing our lab’s experience evaluating and selecting the most appropriate platforms for our lab. We hope that our experience can empower you on your own journey into spatial proteomics.