QIAseq FastSelect is a novel method for ribosomal RNA removal that involves neutralization of the rRNA during the reverse transcription reaction as opposed to the traditional hybridization-capture or hybridization-enzymatic digestion methods – which are commonly used for this purpose. The QIAseq FastSelect method ensures increased ribosomal RNA removal compared to other methods – and only requires a few pipetting steps and a 14-minute incubation. Following the QIAseq FastSelect protocol, the resulting cDNA can be used for RNA-seq library construction using both the traditional dUTP method or the QIAseq Quick-seq method of selective, strand-specific ligation.

Join us for a discussion on how researchers are using QIAseq FastSelect technology with fragmented and high-quality RNA for both short and long-read RNA-seq analysis. Find out how QIAseq FastSelect can speed up and streamline your RNA-seq workflow and allow you to increase the unique reads you generate so you can achieve deeper insights faster. We will also illustrate how QIAseq FastSelect ribosomal RNA removal technology is successfully used for bacterial, mammalian and mixed samples.

Speaker:
Samuel Rulli, Ph.D., Senior Global Product Manager, RNA Profiling, Genomics, QIAGEN