Receptors expressed on the plasma membrane and their interacting partners critically regulate cellular communication during homeostasis and disease, representing main therapeutic targets. Despite its importance in drug development, receptor-ligand proteomics remain a daunting field, partly due to the challenges associated with the study of membrane-expressed proteins. In this webinar, Dr. Bushra Husain will discuss the Conditioned Media AlphaScreen, a new platform for the interrogation of a library consisting of most single transmembrane human proteins. This technology represents a versatile and powerful approach for elucidation of receptor-ligand interactomes, which is essential in understanding basic aspects of plasma membrane protein biology and will ultimately inform the development of novel therapeutic strategies.